Figure 14.

Direct electron transfer anisotropy of a site-specifically immobilized cellobiose dehydrogenase. (A) The enzyme immobilization with a short internal electron transfer path from a catalytic cellobiose dehydrogenase (CDH) domain to a cytochrome (Cyt) mediator domain connected with a flexible spacer. (B) The enzyme immobilization with a long electron transfer path. (C,D) Cyclic voltammograms obtained in the absence (a) and presence (b) of lactose (10 mM) for the enzyme immobilization with the short and long internal electron transfer distances, respectively. (The figure adopted from ref. [168] with permission.)