Figure 2.

Adaptive morphology of epithelial cells on convex geometric structure. (A) Representative fluorescence images of MDCK cell morphology on flat and protruded structures. F-actin (green) was stained with phalloidin-FITC. (B) Averaged cell alignments (∆θ) of the cells on corner of triangle structure (navy), square (orange), and circle (red) shape. (C) Averaged ∆θ of MDCK cells on corner and arm of triangle (navy) and square (orange) structure. (D) Averaged area of the cells on corner of triangle structure, square and circle shape. (E) Averaged area of MDCK cells on corner and arm of triangle and square structure. Number of analyzed cells ($N_{cell}$) for flat surface and corner of triangle, square, circle structure was 1014 and 212, 471, 749, respectively (N = 3). $N_{cell}$ for arm of triangle and square structure were 412 and 580 (N = 3). Error bars in graph indicate the standard error of mean (S.E.M.). One-way ANOVA test with post-hoc Fisher’s least significant difference (LSD) was used. **** p < 0.0001; *** p < 0.001; * p < 0.05; NS, not significant.