Figure 4.
Enhanced vimentin expression on convex geometric structure. (A) Representative fluorescence images of vimentin (red) in MDCK cells on the flat and triangle, square, circle structure. (B) The box plots indicate the total intensity of vimentin per individual cells on corner of triangle (navy), square (orange) and circle (red) structure. The result from flat surface is plotted in gray color as a control (N = 3). (C) Mean relative vimentin intensity of the cells on the geometric structure compared to that on the flat surface. for flat surface and triangle, square, circle structure was 457 and 306, 473, 919, respectively (N = 3). Error bars in graph indicate S.E.M. One-way ANOVA test with post-hoc Fisher’s LSD was used. **** p < 0.0001; ** p < 0.01; NS, not significant.