Table 1.
Viral RNA load per sample in both raw read count summation and normalized fragments per kilobase million (FPKM) average from all virus’ genes combined, obtained in cellular extracts of either macrophages or CRFK cells infected with FIPV, at 2 and 17 h post-infection. Cats #1–6 were healthy cats from the UC Davis cat colony, ages five months (5M) to four years (4Y). In macrophages, there was a limited viral presence/uptake of FIPV at 2 h, or not detected (nd), and no evidence of significant replication at 17 h (cat #6 n/a (no sample available)). In CRFK cells, viral RNA increased several log-fold from 2 to 17 h.
| MØ Origin | Read Counts | Read Counts | FPKM Average | FPKM Average |
|---|---|---|---|---|
| (age of cat) | 2 h | 17 h | 2 h | 17 h |
| cat #1 (2Y) | 727 | 2 | 4.46 × 10−2 | 1.38 × 10−4 |
| cat #2 (2Y) | 375 | nd | 1.82 × 10−2 | nd |
| cat #3 (4Y) | nd | nd | nd | nd |
| cat #4 (5M) | 1798 | 1555 | 9.93 × 10−2 | 9.05 × 10−2 |
| cat #5 (2Y) | 673 | 202 | 3.62 × 10−2 | 8.69 × 10−3 |
| cat #6 (2Y) | 245 | n/a | 1.4 × 10−1 | n/a |
| CRFK 1 | 3715 | 459,899 | 1.38 × 10−1 | 17.4 |
| CRFK 2 | 3 | 4871 | 2.19 × 10−4 | 1.54 × 10−1 |
| CRFK 3 | 808 | 45,208 | 4.71 × 10−2 | 1.70 |
| CRFK 4 | 1576 | 71,746 | 9.04 × 10−2 | 2.91 |