Table 2.

Accurate de novo peptide sequencing with LC-MS/MS of active fraction 4c.

N	de novo Peptide Sequence 1	Conf Score	Hb Transcript 2	Identification	E Value	Homology	GO	Pfam
1	KPIILTNLNVPCQPK	99.0	Hba_21422	Pellino	6 × 10−148	Ancylostoma ceylanicum	Ubiquitin protein ligase	PF04710
2	LLAVHFTVAEGVKNKER	99.0	Hba_15515	Metridin ShK toxin	5 × 10−148	Haemonchus contortus	Toxin activity	PF01549
3	KQEDNEVESPPQQEVPRPR	99.0	Hba_18775	Ion channel	9 × 10−127	Necator americanus	Ion channel activity	PF07885
4	QKEILASTSIAQIVDRVC	99.0	Hba_18934	Hypothetical protein	6 × 10−110	Ancylostoma ceylanicum	Unnamed protein	NI
5	YTFKELTRQEEIVVR	99.0	Hba_19968	CUE domain protein	3 × 10−84	Haemonchus contortus	CUE domain	PF02845
6	MLDEVVPPAGSKLRYIC	99.0	Hba_20430	Unnamed protein	3 × 10−16	Haemonchus placei	Unnamed protein	PF02319

¹ analysis of MS/MS by PEAKS Studio 7.5 Software ² https://parasite.wormbase.org/Heterorhabditis_bacteriophora_prjna13977/Info/Index.