Skip to main content
. 2020 Apr 6;8(2):169. doi: 10.3390/vaccines8020169

Table 2.

Primers used to clone and express recombinant Fc-6x His-tagged EBV gH/gL.

Primer Name Primer Sequence (5’-3’)
Cloning Primers
EBV gH-gL-Fc-His FWD AAAAAGCGGCCGCGCCACCATGCGTGCTGTTGGTGTATTTC
EBV gH-gL-Fc-His REV AAAAAACTAGTGTGTGCTCTTTCTTCATACAGG
Sequencing primers
FC-Hisseqprimer4 GCTTTAATAAGATCTCTAG
FC-Hisseqprimer5 TGCTGGGCACGGTGGGCATG
FC-Hisseqprimer6 GGGTCTTTTCTGCAGAAGCTTG