Figure 2.
Quantitative expression analysis of Schwann cell and nerve fibroblast markers. Relative quantification (2−ΔΔCt) of S100β and p75 (Schwann cell markers), Thy1 (fibroblast marker), soluble NRG1, NRG1α, and NRG1β was evaluated by qRT-PCR. The geometric average of the housekeeping genes ANKRD27 and RICTOR was used to normalize data. Values in the graphics are expressed as mean + SEM (n = 3–4 for each group). ANOVA for repeated measures with Bonferroni’s correction was adopted for statistical analysis; * denotes the significant differences between autograft and chitosan groups at each time point analyzed (*p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001); # denotes the significant differences between autograft group and uninjured control nerves at each time point analyzed (#p ≤ 0.05, ##p ≤ 0.01, and ###p ≤ 0.001); § denotes the significant differences between chitosan group and uninjured control nerves at each time point analyzed (§p ≤ 0.05, §§p ≤ 0.01, and §§§p ≤ 0.001). All data are calibrated to uninjured nerves (whose expression, not shown, is = 1).