Figure 3.

Specific IgG subclasses levels in plasma at the time of the T cell assays. (A,B,C) Subclasses in plasma at the same time as the T cell assays. E. coli-eIL-5- (A), HEK-eIL-5- (B), and CuMV_TT- specific (C) IgG1, IgG4/7, and IgG5 were measured in the plasma of unvaccinated (−) control horses (grey), first (blue), second (red), or third (green) year vaccinated (+) horses by ELISA. OD405 value of the individual horses with the mean and SEM for each group. The Mann–Whitney U test was used to compare the difference between the groups within each subclass. (D,E) IgG1 and IgG4/7 were measured in the serum of vaccinated horses against CuMV_TT (D) and HEK-eIL-5 (E) by ELISA, mean with SEM. The time points measured: before the initial vaccinations (V), after the initial vaccinations (A), at the time of the annual boost at the 1st year (V1), 2–4 weeks after the boost at the 1st year (A1), at the time of the annual boost at the 2nd year (V2), 2–4 weeks after the boost at the 2nd year (A2), at the time of the annual boost at the 3rd year (V3), and 2–4 weeks after the boost at the 3rd year (A3). Black arrows indicate the initial vaccinations and the gray arrows the yearly booster. ** p < 0.01; **** p < 0.0001.