Figure 1.

Sample processing time affects plasma MMP9, HK1, and RAS expression levels. Plasma MMP9, HK1, and RAS expression levels were measured at different time points: 30 min, 2 h, 4 h, and 24 h after blood draw. MMP9 expression was measured by luminex xMAP technology. Ras and HK1 expression were measured by Western blot. N represents number of independent biological replicates. Statistical significance was measured by Student’s t-test when n ≥ 3; * represents p < 0.05; error bars represents mean ± standard error of mean. Target protein expression levels were normalized to the 30 min time point to determine change in expression with time.