Figure A1.

Immunoblot reactivity for Borrelia burgdorferi sensu lato (Bbsl). Immunoblot strips with recombinant Borrelia antigens were incubated with representative patient serum samples, as described in Section 2. Blots were interpreted according to IGeneX criteria (see text). P—positive control; N—negative control; G—Bbsl immunoblot immunoglobulin G (IgG); M—Bbsl immunoblot IgM; Lane 1—B. burgdorferi B-31 positive IgM and IgG; Lane 2—B. mayonii positive IgG; Lane 3—B. speilmanii positive IgG; Lane 4—B. californiensis positive IgM and IgG; Lane 5—mixed infection B. mayonii and B. californiensis positive IgM; Lane 6—B. burgdorferi European species positive IgM and IgG; Lane 7—Bbss and B. burgdorferi European species positive IgM.