Table 1.
Assessment of biochemical parameters in IV-infected SCID mice.
| Treatment | PBS | γ-Irradiated M. brumae |
M. brumae | BCG | p Value | ||||
|---|---|---|---|---|---|---|---|---|---|
| Parameters | Mean ± SD | Range | Mean ± SD | Range | Mean ± SD | Range | Mean ± SD | Range | |
| ALT (U/L) a | 17.86 ± 5.82 | (11.30–30.80) | 17.89 ± 6.39 1 | (12.20–31.50) | 16.74 ± 3.05 1 | (14.00–22.60) | 61.53 ± 36.81 3 | (27.80–100.80) | < 0.001 |
| AST (U/L) b | 73.50 ± 38.43 | (42.00–163.00) | 51.29 ± 10.81 1 | (42.00–74.00) | 65.86 ± 17.30 1 | (49.00–88.00) | 326.00 ± 74.62 4 | (249.00–428.00) | < 0.0001 |
| Creatinine (mg/dL) | 0.27 ± 0.01 | (0.25–0.29) | 0.27 ± 0.02 | (0.24–0.28) | 0.28 ± 0.01 1 | (0.27–0.30) | 0.23 ± 0.04 5 | (0.17–0.28) | < 0.05, < 0.01 * |
| Iron (µg/dL) | 224.11 ± 40.55 | (158.40–274.10) | 218.26 ± 43.96 | (156.40–276.70) | 203.37 ± 9.89 2 | (188.10–214.50) | 119.84 ± 31.025 | (88.00–157.70) | < 0.01 #, < 0.001 |
| Alkaline Phosphatase (mmol/L) | 72.54 ± 4.71 | (67.20–78.46) | 77.69 ± 11.24 | (68.17–100.46) | 80.10 ± 7.99 1 | (66.90–90.02) | 76.70 ± 11.75 6 | (68.39–85.00) | ns |
| Glucose (mg/mL) | 271.98 ± 26.89 | (232.20–317.40) | 285.51 ± 45.10 | (233.70–352.00) | 269.60 ± 44.31 1 | (213.30–340.10) | 121.37 ± 3.55 3 | (118.20–125.20) | < 0.0001 |
| Potassium (mmol/L) | 4.64 ± 0.74 | (3.61–5.90) | 4.89 ± 0.70 | (3.96–5.75) | 4.27 ± 0.55 | (3.65–5.10) | 5.95 ± 0.66 5 | (5.35–6.84) | < 0.05, < 0.01 &, < 0.0001 ^ |
| Sodium (mmol/L) | 147.00 ± 1.61 | (143.70–148.80) | 146.33 ± 1.32 | (144.50–148.10) | 148.88 ± 1.64 | (146.50–151.10) | 149.14 ± 1.86 5 | (147.00–151.20) | < 0.05 |
| Urea (mg/dL) | 47.01 ± 7.83 | (33.10–53.60) | 44.21 ± 9.36 | (34.20–60.50) | 44.87 ± 8.61 1 | (35.50–60.60) | 68.65 ± 16.27 4 | (55.60–92.20) | < 0.01 |
| Albumin (g/dL) | 2.87 ± 0.12 | (2.72–3.10) | 2.82 ± 0.09 | (2.61–2.89) | 2.82 ± 0.10 1 | (2.71–3.00) | 2.14 ± 0 7 | − | ns |
| Total Protein (g/dL) | 4.88 ± 0.19 | (4.57–5.20) | 4.77 ± 0.18 | (4.38–4.93) | 4.84 ± 0.12 1 | (4.69–5.04) | 4.66 ± 0 7 | − | ns |
Table shows mean and standard deviation (± SD) of different biochemical parameters in PBS-, γ-irradiated M. brumae-, M. brumae- and BCG-IV-infected mice. Abbreviations: a ALT (Alanine transaminase); b AST (Aspartate transaminase). Data are expressed as mean ± SD and the range of each parameter for each experimental group. N = 8 except for indicated parameters of experimental groups that had insufficient volumes of sample: 1 n = 7; 2 n = 6; 3 n = 3; 4 n = 4; 5 n = 5; 6 n = 2 and 7 n = 1). Significant differences were established by one-way ANOVA comparing the experimental groups versus BCG except: * (M. brumae vs. BCG), # (M. brumae vs. BCG), & (PBS vs. BCG) and ^ (M. brumae vs. BCG).