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. 2020 May 29;11(6):603. doi: 10.3390/genes11060603

Figure 3.

Figure 3

Mucus characteristics in HNE organoids, representative findings from CF and non-CF organoids are shown. (A) MUC5B granules in G551D/N1303K organoid. Scale = 50 µm. (B) Muc5B whole-mount IF showing a bundled mucin pattern in F508del/R117H-5T organoid. Scale = 50 µm. (C) MUC5AC fixed cross-section showing a diffuse mucin pattern in a non-CF organoid. Scale = 50 µm. (D) Top panel is the maximum projection of the 3-D hemisphere in the bottom panel. MUC5B is seen entering the lumen (arrow) through the apical surface in a non-CF organoid. Scale = 500 µm. (E) Brightfield microscopy showing a clump of cellular debris and/or mucus in the lumen in a non-CF organoid. Scale = 100 µm. (F) µOCT still of probable mucus in a non-CF organoid. (E,F) correspond to Videos S1 and S2. E = epithelium L = lumen M = mucus.