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. 2020 Jun 8;11(6):628. doi: 10.3390/genes11060628

Figure 1.

Figure 1

Magi1 guide and donor pair sequences. Four guide/donor pairs were used to alter serine 733 in the mouse Magi1 gene to alanine. The M67, M79 and M91 guides had donors containing the variable region of the guide sequence (except guides had U in place of T) and these guide RNA regions are shown with the adjacent PAM in lower case. We also used the M79 guide with another donor, M79R donor, containing sequence complementary to the variable region of the M79 guide. Donor point mutations are shown out of line with the main donor sequence.