Figure 7.

Sanger sequences of blastocysts integrating two different mutations into the same position on homologous/sister chromosomes. In most cases where two donors were simultaneously injected, either the D donor’s sequence or the A donor’s sequence was integrated. The WT sequence (a) was changed from TC to GA (two-point mutations, (b,c)) to produce the D amino acid and from T to G (one-point mutation, (d)) to produce the A amino acid. The two peaks for the D mutation are of a similar height, regardless of whether all (b) or some (c) of the bases replace the WT sequence. In one case, the two peaks of the integrated point mutations were of clearly different heights and the WT peaks at the same position were inversely of different heights (e) implying that both donor sequences were integrated into the genomic DNA in the same blastocyst. The sequence for each blastocyst is shown above its chromatogram. The WT sequence is shown at the very top.