Table 2.

Sequence, size, GC content, melting temperature (T_m) and change in free energy of hybridization (ΔG) of the oligonucleotide probes specifically designed to target each bacteria (E. coli, Klebsiella sp., S. aureus, S. uberis, and S. agalactiae) and the negative control. The melting temperature and Gibbs energy were calculated by the nearest-neighbor model.

Target	$\mathbf{Sequence} (5\prime \to 3\prime )$	Size (bp)	GC%	Tm (°C)	ΔG (kcal/mol)
E. coli	GAGCAAAGGTATTAACTTTACTCCC	25	40	53.5	−43.90
Klebsiella sp.	CACATCCGACTTGACAGA	18	50	51.6	−31.44
S. aureus	CACTTTTGAACCATGCGGTTCAAAATATTATCC	33	36.4	58.7	−61.40
S. uberis	GAACTATGGTTAAGCCACA	19	42.1	49.5	−33.17
S. agalactiae	AACTAACATGTGTTAATCACTCTTATGC	28	32.1	53.8	−44.59
Neg. control	GCCTGGCGATACCGCTGTTA	20	60	57.1	−