Figure 1.

Anti-schistosome cholinesterase (SmChE) antibodies inhibit ChE activity in schistosomula, which leads to a decreased parasite viability. Newly transformed schistosomula (1000/treatment) were incubated in Dulbecco’s Modified Eagle Medium (DMEM) in the presence of anti-SmChE IgG and incubated at 37 °C in 5% CO₂. Naïve IgG served as a negative control. (A) AChE activity 2 h after treatment. (B) BChE activity 2 h after treatment. (C) Schistosomula viability 2 h after treatment. (D) AChE activity 14 h after treatment. (E) BChE activity 14 h after treatment. (F) Schistosomula viability 14 h after treatment. Data represents the mean ± SEM of two biological and three technical replicates. Significance (relative to the naïve IgG control) determined by the student’s t test, where ** p ≤ 0.01 and *** p ≤ 0.001.