Figure 5.

Effect of SmChE vaccination on glycogen storage in, and the size of, S. mansoni adult worms. (A) Triton-X-100 extracts were made from five pairs of worms freshly perfused from each vaccinated or control group and the glycogen content in these extracts was measured. Plotted data are the average of triplicate biological and technical experiments ± SEM. (B) Worm sizes (mm) were assessed by randomly selecting and measuring (ImageJ) at least 20 worms from each group. Significance and percent reductions (if any) for both parameters were measured relative to the control group. Differences for both experiments were measured by the student’s t test, where ** p ≤ 0.01 and *** p ≤ 0.001.