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. 2020 Jun 23;9(6):1528. doi: 10.3390/cells9061528

Figure 10.

Figure 10

CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats- associated protein 9) -induced disruption of green fluorescence protein (GFP) expression in the daughter cells of single microinjected aRGCs in organotypic slices of the telencephalon of Tis21::GFP mouse embryos. (a) Scheme of the Cas9/gRNA complex microinjection. (b) Reconstruction of optical sections with maximum intensity projections for daughter cells of single aRGCs microinjected with either Cas9/control gRNA (top) or Cas9/gGFP (bottom) revealed by Dx-A555 immunofluorescence (magenta); cell 1, aRGC daughter; cell 2, BP daughter. Dashed lines depict ventricular surface. Scale bars, 20 μm. (c) Single optical sections of cells 1 and 2 shown in (b), showing the effects of Cas9 and control gRNA (top) or gGFP (bottom) on GFP expression. Scale bars, 5 μm. (d) Quantification of the proportion of daughter cells (Dx-A555+) of microinjected cells showing GFP expression 24 h after control (Con, white) or gGFP (black) microinjection. (* p < 0.05, Fisher’s test) Reprinted with the permission of [147].