Figure 12.

Microinjectrode system and its application. Briefly, a thin microelectrode passes through a 32 G cannula (OD: 236 m) which is connected to a T-junction via a ferrule. The electrode goes into a T-junction and a polyimide-coated glass tube with the terminal soldered to a gold pin. The polyimide tubing, gold pin, and ferrule are all pasted together. The middle part shows cross-sections through different parts of microinjectrode, i.e., the top ferrule, middle T-junction and bottom the cannula. An enlarged view of the microelectrode and cannula tips shows their relative position and size. A sample experiment is also displayed with single-neuron recording, electrical microstimulation and microinjection being performed in the frontal eye field (FEF). The single-neuron waveforms (black traces) segregated from background (gray traces) are also presented. Reprinted with the permission of [149].