Figure 15.

Immunostaining images of single-cell electroporated Purkinje cells small interfering RNA (siRNA) against calcium/calmodulin-dependent protein kinase ß (CaMKIIß) or 14-3-3η). SCEP was done at 11 days in vitro (DIV). The cell fixation was performed on day 7 (a,c) or day 14 (b,d) post electroporation (18 or 25 DIV, respectively) and double fluorescent immunostaining against CaMKIIß (green in a,b) and calbindin-D-28 K (CBD28K) (red in a,b) or 14-3-3η (green in c,d) and IP₃R (red in c,d) was performed. Therefore, 1, 2 and 3 correspond to green, red and merged stains respectively. CaMKIIß or 14-3-3η signals decreased in electroporated Purkinje cells (arrows), but not in nearby non-electroporated Purkinje cells (asterisks). It is noteworthy that CaMKIIß and 14-3-3η expression was present for both Purkinje cells and granule cells. Scale: 20 µm. Reprinted with permission from [168].