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. 2020 Jun 11;9(6):1454. doi: 10.3390/cells9061454

Figure 3.

Figure 3

The impact of Parkin overexpression and sepsis on skeletal muscle catabolic signaling. (A) qPCR analysis of the mRNA expression of genes regulating apoptosis in the gastrocnemius (GAS) muscles of Sham and CLP animals injected with either AAV-GFP or AAV-Parkin. (B) qPCR analysis of autophagy-related gene expression in the gastrocnemius (GAS) muscles of Sham and CLP animals injected with either AAV-GFP or AAV-Parkin. Gaba. refers to Gabarapl1. (C) Immunoblot detection of SQSMT1(p62), BNIP3, LC3I/LC3II and GAPDH. (D) Quantification of SQSMT1 (p62) content. (E) Quantification of BNIP3 protein content. (F) Quantification of LC3I and LC3II protein content, as well as the LC3II to LC3I ratio. (G) qPCR analysis of Fbxo32 (Atrogin-1) and Trim63 (MuRF1) gene expression levels in the GAS muscles of Sham and CLP animals injected with either AAV-GFP or AAV-Parkin. 1 = Sham-AAV-GFP; 2 = Sham-AAV-Parkin; 3 = CLP-AAV-GFP; 4 = CLP-AAV-Parkin. Data are presented as mean ± SEM. (n = 6–9/group, * = statistically significant; ns = not statistically significant).