Figure 2.

Validation and quantitative analysis of cell events in wells from scanned images. (a) Images were obtained by scanning 96-well plates after the individual cells were sorted through FACS for 30 min. Enlarged images show double cells in a well (right). (b) Images were obtained by scanning a microfluidic device within 10 min after the single cell was sorted (left). The white frame from the left image was enlarged (middle), showing merge, fluorescence, and phase images. Two arrows in each well indicate double cells. Enlarged images show merge and phase images of double cells (right). (c) Quantitative analysis of cell events per well after single-cell sorting using the limiting dilution method, the SCC device, and FACS. * p < 0.05., ** p < 0.005. Student’s t-test. n = 4, two independent experiments.