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. 2020 Apr 7;8(2):170. doi: 10.3390/vaccines8020170

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Recognition of hAd5-ZKV antigens by polyclonal human anti-ZIKV antibody response. Uninfected and ZIKV Vero cells, as well as uninfected and hAd5-ZKV-infected 293 cells, were probed with human serum from subjects that had a naturally acquired ZIKV infection, demonstrating recognition of ZIKV E antigens by the human immune response. (A) Serum from a flavivirus-naïve individual was used as a control, with staining for Ad5 hexon protein apparent. (B)–(D) Serum from three human positive subjects after 9–12 months after a ZIKV-positive test showed staining for E and hAd5 hexons.