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. 2020 Jun 16;9(6):1475. doi: 10.3390/cells9061475

Figure 2.

Figure 2

Streptonigrin and nutlin-3a increase p53 protein levels in a dose-dependent manner. (A) ACHN (left) and CAKI-1 (right) cells were treated with streptonigrin (which inhibits TGase 2-p53 binding) or nutlin-3a (which inhibits MDM2-p53 binding) for 24 h. Doxorubicin (1 μM) was used as a positive control. (B) Image J analysis of the western blots in A. (C) The apoptosis assay was performed in the presence/absence of streptonigrin or nutlin-3a and was monitored by RealTime-Glo™ annexin V under the conditions outlined in A. Cumulative data from three independent experiments are shown (mean ± SD; n = 3). **p < 0.01 and ***p < 0.001.