Figure 4.

Harmol hydrochloride binds to AR. U2OS-hAR-ARE-Luc cells were incubated with 0.3 nM [³H]-labeled R1881 at 37 °C for 3 h in the absence or presence of enzalutamide, harmol hydrochloride (0.01–10 µM), or unlabeled R1881 (0.01–300 nM). Non-specific binding was determined in the presence of 300 nM unlabeled R1881. Specific binding was calculated by subtracting non-specific binding from total binding. Results were plotted as % of maximal [³H]-labeled R1881 binding as a function of drug concentrations (n = 3 ± SEM).