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. 2020 May 27;9(6):1335. doi: 10.3390/cells9061335

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorogold (FG)⁺ retinal ganglion cells (RGC) at 21 days after grafting in retinal wholemounts from: (A) CON/intact, CON/ivit_S/ONT, ivit_ASN/ONT, ivit_S/ONT_ASN, ivit_S/ONT_CSN, ivit_CSN/ONT, ivit_CSN/ONT_ASN, ivit_ASN/ONT_CSN, and ivit_CSN/ONT_CSN groups. Note that the ivit_CSN/ONT_CSN group show the greatest RGC neuroprotection (scale bars = 20 μm). (B) Quantification of numbers of FG⁺ RGC in retinal wholemounts confirms that RGC survival is increased in all ivit_CSN paradigms with the greatest survival observed in the ivit_CSN/ONT_CSN group. n = 16 retinae/group; ** p < 0.001; *** p < 0.0001, ANOVA.