Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 2;11(6):613. doi: 10.3390/genes11060613

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Transcriptional activity of the combination of AtIDD proteins and REPRESSOR of ga1-3 (RGA) or SHR + SCARECROW (SCR) on several promoters. (A) The location of the IDD binding sequence candidates. IDDBS: TTTGTC(G/C)(T/C)(T/a)(T/a)T; MBPBS: TTGTCT; IDDBS-like1: 11bp sequence containing GTC(G/C), with more than seven nucleotides identical to IDDBS; IDDBS-like2: 11bp sequence containing GTC(G/C), with less than six nucleotides identical to IDDBS. (B) Schematic diagram of reporter and effector plasmids used in transient assays. CaMV35p: CaMV35S promoter, NOS: NOS terminator. The promoter regions of (C) SCR, (D) SCL3, (E) GA3ox1, (F) PIN1, and (G) YUC5 were used for reporter construction. As a control, an empty vector was used, and all luciferase reporter gene (LUC) activities were expressed relative to this control (value set at 1). Values shown are the average of results from three or four independent experiments. Error bars represent standard deviation (SD). Asterisks represent significantly different activity from those of AtIDD alone (* p < 0.05, ** p < 0.01).

Transcriptional activity of the combination of AtIDD proteins and REPRESSOR of ga1-3 (RGA) or SHR + SCARECROW (SCR) on several promoters. (A) The location of the IDD binding sequence candidates. IDDBS: TTTGTC(G/C)(T/C)(T/a)(T/a)T; MBPBS: TTGTCT; IDDBS-like1: 11bp sequence containing GTC(G/C), with more than seven nucleotides identical to IDDBS; IDDBS-like2: 11bp sequence containing GTC(G/C), with less than six nucleotides identical to IDDBS. (B) Schematic diagram of reporter and effector plasmids used in transient assays. CaMV35p: CaMV35S promoter, NOS: NOS terminator. The promoter regions of (C) SCR, (D) SCL3, (E) GA3ox1, (F) PIN1, and (G) YUC5 were used for reporter construction. As a control, an empty vector was used, and all luciferase reporter gene (LUC) activities were expressed relative to this control (value set at 1). Values shown are the average of results from three or four independent experiments. Error bars represent standard deviation (SD). Asterisks represent significantly different activity from those of AtIDD alone (* p < 0.05, ** p < 0.01).