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. 2020 May 18;8(2):229. doi: 10.3390/vaccines8020229

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Porcine monocyte-derived dendritic cells (MoDCs) treated with Nano-11-KAg-poly(I:C) increased the cytokine mRNA expression. Porcine PBMCs-derived MoDCs were treated with medium (control), Nano-11, Nano-11-KAg, Nano-11-poly(I:C), and Nano-11-KAg-poly(I:C) for 24 h and different cytokine mRNA expressions were analyzed by qRT-PCR. The fold-change in mRNA expression levels of (A) TNF-α; and (B) IL-1ß was determined. Each bar is the mean ± SEM of three pigs and the data were analyzed by one-way ANOVA followed by Tukey’s post hoc comparison test. Asterisk refers to statistical difference between the indicated treatment groups (** p < 0.01 and *** p < 0.001).