Figure 4.

Recall IFN-γ secreting lymphocyte response in Nano-11 and commercial influenza vaccinated and virus challenged pigs. PBMCs isolated at post-challenge day six were stimulated with vaccine H1N2-OH10 SwIAV, immunostained and analyzed for the frequency of IFN-γ secreting (A) T-helper/memory cells (CD3⁺CD4⁺CD8α⁺); and (B) γδ T cells by flow cytometry. Data represent the mean value of four to five pigs ± SEM. Statistical analysis was carried out using one-way analysis of variance followed by Tukey’s post hoc comparison. Asterisk refers to statistical difference between the two indicated groups (** p < 0.01). Ch—Challenge.