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. 2020 Jun 6;8(2):283. doi: 10.3390/vaccines8020283

Figure 1.

Figure 1

Chimeric protein (N-terminus of flagellin-truncated, conserved region of P. multocida lipoprotein E (nFliC-tplpE)) construction and protein analysis. (a) Previously solved 3D structure of the full-length flagellin (Protein Data Bank ID: 1UCU) as illustrated by the EzMol server is shown containing domains D0, D1, D2 and D3. (b) Predicted 3D structure of nFliC-tplpE using the Phyre2 server. (c) Cloning site for nFliC-tplpE into the pET32a expression vector. (d) SDS-PAGE and (e) Western blot analyses of expressed recombinant proteins. SDS-PAGE of purified (f) nFliC-tplpE and (g) tplpE proteins. GS: glycine-serine linker.