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. 2020 Jul 3;11:1235. doi: 10.3389/fmicb.2020.01235

FIGURE 4.

FIGURE 4

Impact of the mutations on the structure of PSTVd-RG1. (A) The deletion or insertion of an adenosine at position 55 results in a decrease and an increase in the size of the A-motif, respectively. A nucleotide in bold indicates an insertion. (B) The deletion of the adenosine at position 118 reduces the size of the loop. (C) The substitution of adenosine for the guanosine located at position 168 (in green) makes a single larger loop in the TR domain instead of the 2 smaller ones that are present in the wild type. (D) The structure of the hairpin I (HPI) is affected by an insertion of one nucleotide (in bold) at position 253. (E) The structure of both the hairpin I (HPI) and Loop E are affected by an insertion of one nucleotide (in bold) at position 254. (F) The deletion or insertion of a uracil at position 296 resulted in either an increase or a decrease in the size of the A-motif. (G) The deletion of the guanine located at position 353 increases the size of the loop in the TL domain, whereas the substitution G353A is a covariation (i.e., G:U base pair becomes an A:U base pair). Nucleotide insertion, deletion and substitution are shown in black, red, and green, respectively.