Figure 4.

Expansion of lymphatic endothelial cells in the lungs of DMXAA treated mice. (a) Left Panel: Flow cytometry analyses showing CD45- CD326- endothelial cells (red arrow) in the live gate. Right panel: Gating strategy for lymphatic (CD31+ and Podoplanin+) and vascular (CD31+ and Podoplanin-) endothelial cells; (b) Lymphatic and vascular endothelial cells in lungs from DMXAA treated mice and vehicle-treated controls expressed as the frequency of the CD45- CD326- gate. Data are pooled from two independent experiments and represent mean + SEM of n = 9 mice/group. An unpaired two-tailed t-test was used to determine statistical significance, and p < 0.05 was considered significant.