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T-cell immunogenicity of prime-boost vaccination determined by intracellular cytokine staining (ICS). CD-1 mice (n = 10 per group) were primed with either triFilo (2A) vaccine or mix of monovalent controls; subsequently, all mice were boosted with multiMVA. Cytokine levels determined by ICS for T cells two weeks post boost vaccination, with four weeks prime-boost interval. (a) IFN-γ+ CD8+ T cells. (b) CD8+ T cells expressing IFN-γ, TNF-α, and IL-2.
