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T cell responses in splenocytes and CLNs induced by intranasal immunizations with rH7 proteins. Splenocytes and CLNs collected from immunized mice were cultured and stimulated with rH7 proteins. Culture supernatants were analyzed using ELISA to measure (A,B) IFN-γ, (C,D) IL-4, and (E,F) IL-17A cytokine levels to determine H7-specific Th1, Th2, and Th17 cell activation, respectively. Statistical analysis consisted of one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
