Table 1.

Cell characteristics of glioblastoma induction.

Ref.	Year	Tumor Cell	Source of Cells	Medium Culture	Supplement	Cell Modifications
Wu et al. [50]	2019	GL261	Mouse	DMEM	10%FBS	NA
Sousa et al. [32]	2019	U87-MG	Human	DMEM	10%FBS; 10 μg/mL Blast	Luciferase expression
Clavreul et al. [34]	2018	U87-MG	Human	DMEM-HG	10%FBS; 1% antibiotics	NA
Sun et al. [51]	2017	C6	Rattus norvegicus	DMEM	10%FBS; 2 mM Glut; 100 U/mL Pen; 100 mg/mL Strep	NA
Séhédic et al. [52]	2017	U87-MG	Human	DMEM-HG	10%FBS; l-Glut; 10 U/mL Pen; 10 mg/mL Strep; 25 μg/mL Amp-B	Expression of CXCR4 and RFP
Lu et al. [53]	2017	U87-MG	Human	DMEM	10%FBS; 1% Pen/Strep	NA
Xu et al. [54]	2016	C6	Rattus norvegicus	DMEM-HG	FBS	NA
Wang et al. [55]	2016	U87-MG	Human	DMEM	10%FBS	Luciferase expression
Lin et al. [56]	2016	U87-MG	Human	DMEM	10%FBS; 1% antibiotics	Luciferase expression
Lin et al. [57]	2016	U87-MG	Human	MEM	10%FBS; 2 mM l-Glut; 100 U/mL Pen; 100 mg/mL Strep; 1 mM SP; 1 mM NAA	Luciferase expression
Kuang et al. [58]	2016	U87-MG	Human	NR	NR	Luciferase expression
Hu et al. [59]	2016	U87-MG	Human	DMEM	FBS; Pen/Strep	NA
Hu et al. [60]	2016	C6	Rattus norvegicus	NR	NR	NA
Banerjee et al. [61]	2016	C6	Rattus norvegicus	DMEM	10%FBS; 1 mM Glut; 100 U/mL Pen; 100 ng/mL Strep	NA
Zhang et al. [45]	2015	U251-MG	Human	MEM-EBSS	10%FBS	NA
Feng et al. [62]	2015	U87-MG	Human	DMEM-HG	10%FBS; 100 U/mL Pen; 100 µg/mL Strep	NA
Costa et al. [44]	2015	GL261	Mouse	DMEM-HG	10%FBS; 100 U/mL Pen; 100 µg/mL Strep; 100 mM HEPES; 12 mM NaHCO3	NA
Bechet et al. [63]	2015	U87-MG	Human	NR	NR	NA
Gao et al. [41]	2014	C6	Rattus norvegicus	DMEM-HG	FBS	Expression RFP
Wojton et al. [64]	2013	U87-MG	Human	DMEM	10%FBS; 100 U/mL Pen; 10 mg/mL Strep	U87ΔEGFR; U87ΔEGFR-Luc
Janic et al. [65]	2012	U251-MG	Human	DMEM	10%FBS	NA
Day et al. [48]	2012	U373-MG	Human	RPMI 1640	10%FBS; 1% Glut	Luciferase expression
Verreault et al. [43]	2011	U251-MG	Human	DMEM	10%FBS; 1% l-Glut; 1% Pen; 1% Strep	NA
Agemy et al. [66]	2011	U87-MG	Human	DMEM-F12	10%FBS; 1% Glut; 1% Pen; 1% Strep	Luciferase expression
		005	Mouse	DMEM-F12	1%N2; 20 ng/mL FGF-2; 20 ng/mL EGF; 40 μg/mL heparin	Luciferase expression
		Spheres#	Human	DMEM-F12	l-Glut; 0.3% Gluc; 50 μg/mL Pen/Strep; 0.1 mg/mL Apo-transf; 20 nM Prog; 30 nM Na2SeO3, 60 μM Put; 25 μM/mL Ins; 3 mM NaHCO3; 10 mM HEPES; 20 ng/mL EGF, 10 ng/mL LIF; 20 ng/mL FGF	NA
		H-RasV12-shp53 lentivirus	NA	NA	NA	Luciferase expression
Ding et al. [67]	2010	U87-MG	Human	MEM	10% FBS	NA
Hekmatara et al. [68]	2009	101/8*	Rattus norvegicus	NA	NA	NA
Saito et al. [38]	2006	U87-MG	Human	MEM	10% FBS; 100 U/mL; 0.1 mg/mL Strep	NA

Abbreviations—Ref.: reference; NA: not applicable; NR: not reported; DMEM: Dulbecco’s modified Eagle’s medium; DMEM-HG: DMEM-high glucose; MEM: minimum essential medium; MEM-EBSS: Eagle’s minimum essential medium with Earle’s balanced salts; RPMI 1640: Medium Roswell Park Memorial Institute 1640; DMEM-F12: Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12; FBS: Fetal Bovine Serum; Blast: Blasticidin; Glut: Glutamine; Pen: Penicillin; Strep: Streptomycin; l-Glut: l-Glutamine; Amp-B: Amphotericin-B; SP: Sodium pyruvate; NAA: Nonessential Amino Acids; HEPES: (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); NaHCO₃: Sodium bicarbonate; EGF: Epidermal Growth Factor; LIF: Leukemia Inhibitory Factor; FGF: Fibroblast Growth Factors; N2: N2 supplement; Gluc: Glucose; Apo-transf: Apo-transferrin; Prog: progesterone; Na₂SeO₃: Sodium Selenite; Put: putrescine; Ins: Insulin; CXCR4: C-X-C chemokine receptor type 4; RFP: Red Fluorescent Protein; U87ΔEGFR: Normal expression of the epidermal growth factor receptor; U87ΔEGFR-Luc: Normal expression of the epidermal growth factor receptor marked luciferase. Note—*The rat 101/8 glioblastoma is an orthotopic model initially generated by the injection of α-dimethylbenzanthracene (α-DMBA) into the cerebellum of Wistar rats followed by serial transplantation of tumor tissues into the hemisphere of Wistar rats; #Human GBM spheres lines BT37, BT70, and BT7.