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. 2020 Jun 11;8(2):299. doi: 10.3390/vaccines8020299

Figure 1.

Figure 1

Post-vaccination Salmonella antigens’ specific IgG and IgA antibody responses. Broilers were vaccinated with CS(OMP+FLA)-F, CS(OMP+FLA)-M and CS(OMP+FLA)-F&M orally at age 3 days, with each dose containing 10 µg (OMP+FLA), followed by a booster dose at age 3 weeks. The control birds received mock saline, 10 µg of Sol.Ag (OMP+FLA) and commercial live Salmonella vaccine orally on the same days of our candidate vaccines schedule. Blood and cloacal swab samples collected at week 5 (2 weeks after booster) post-vaccination were analyzed for detection of specific IgG and IgA antibodies, respectively, by ELISA. Serum samples were analyzed for IgG antibody response against (A) OMP and (B) FLA protein. Cloacal swabs were analyzed for IgA antibody response against (C) OMP and (D) FLA protein. Data are presented as the mean ± SEM of 6 to 13 birds per group. Significant differences were determined by one-way ANOVA followed by Tukey post-hoc test between each of the groups. Only significant differences are labelled in the figures (* p < 0.05, ** p < 0.01, and *** p < 0.001).