Figure 2.

Salmonella antigen-specific IgA antibody in vaccinated and infected birds. Broilers were vaccinated as described in figure legend 1, challenged at the age of 5 weeks with Salmonella Enteritidis, and euthanized 10 days post-challenge, and samples were collected for specific IgA antibody analysis by ELISA. The anti-FLA IgA antibody response in (A) cloacal swab, (C) small intestine wash, and (E) bile samples. The anti-OMP IgA antibody response in (B) cloacal swab, (D) small intestine wash, and (F) bile samples. Data are presented as the mean ± SEM of 6 to 13 birds per group. Significant differences were determined by one-way ANOVA followed by Tukey post-hoc test between each of the groups. Only significant differences are labelled in the figures (* p < 0.05, ** p < 0.01, and *** p < 0.001).