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. 2020 May 30;8(2):262. doi: 10.3390/vaccines8020262

Figure 1.

Figure 1

Deletion of the I329L gene from the OURT88/3 and Georgia 2007/1 genomes. (a) The position and direction of the I329L gene and neighbouring genes on the wild-type OURT88/3 genome are shown on the top panel. Arrows indicate the direction in which the genes are read. The bottom panel shows details of the construction of the I329L gene deletion. The β-Glucoronidase (GUS) gene under control of the African swine fever virus (ASFV) B646L gene promoter and flanked by loxP sites was used to replace the I329L gene, excluding the portion encoding residues 1 to 113. This fragment was retained to avoid disrupting the overlapping I78R gene, which is read from the opposite strand. (b) Analysis of genomic viral DNA by PCR. Viral DNA was extracted from wild-type (wt) and delta I329L (Δ) viruses. Specific fragments were amplified by PCR and the products were analysed by agarose gel electrophoresis. For OURT88/3, the fragments encompassing the flanking regions of the I329L deletion are shown and the expected fragment sizes are indicated below each lane. For Georgia 2007/1, a fragment within the deletion was obtained for the wt but not for the deletion mutant virus as expected.