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. 2020 May 30;8(2):262. doi: 10.3390/vaccines8020262

Figure 3.

Figure 3

Expression of type I interferon (IFN) in macrophages infected with wild-type and I329L deletion mutants. Panel (a) shows IFN-β transcripts relative to GAPDH. Pig alveolar macrophages (PAMs) were mock infected or infected with wild-type OURT88/3 or OURT88/3Δ329L viruses at an multiplicity of infection (MOI) of 1. At different times post-infection (x-axis), RNA was extracted and copy numbers for IFN-β and GADPH were quantified by quantitative real time PCR (qPCR) (y-axis). Panel (b) shows IFN-α detected in supernatants from porcine bone marrow cell cultures infected with wild-type or I329L gene-deleted ASFV isolates. PBM cells were mock infected or infected with wild-type Georgia or OURT88/3 isolates and I329L gene deletions from these viruses as indicated on the x-axis at an MOI of 1. At 16 h, post-infection levels of IFN-α were assayed and are shown as units per/mL on the y axis. The results shown are averages from infections of cells from 3 different outbred pigs. Asterisks indicate statistically significant differences between the indicated samples (** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001).