Figure 4.
A-3A and A-HSP70 mediated-immune response in pigs. For the pig experiments, FMD antibody-seronegative animals were used (the pigs were 10–12 weeks old). Pigs were divided into 3 groups (n = 5/group). Pigs were administrated oil emulsion free-test vaccines, including A-HSP70 antigen alone, or combined A-3A and A-HSP70 antigens based on their positive control composition. A positive control group of pigs received 15 µg (1 dose for cattle and pig use) naïve A antigen, without (w/o) oil emulsion, 10% Al(OH)3, and 150 µg Quil-A. The vaccination was performed twice at a 28-day interval, and 1 ml vaccine (1 dose) was injected via a deep intramuscular route at the animals’ necks. Blood samples were collected at 0, 7, 14, 28, 42, 56, 70, and 84 dpv in pigs for the serological assays and at 0, 14, 28, and 56 dpv for PBMC isolation. (A–C) represent: (A) the strategy for this study; (B) antibody titers by SP A ELISA; (C) VN titers. The data represent the means ± SEM of triplicate measurements (n = 5/group). Statistical analyses were performed using two-way ANOVA with Bonferroni correction. * p < 0.05; ** p < 0.01; *** p < 0.001.