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. 2020 May 28;8(2):254. doi: 10.3390/vaccines8020254

Figure 5.

Figure 5

A-3A and A-HSP70 induced co-stimulatory molecule and cytokine gene expression in porcine PBMCs. Porcine peripheral blood mononuclear cells (PBMCs) isolated from the whole blood of vaccinated pigs (n = 5/group) individually, as described in Figure 4, were used for the qRT-PCR assay. Gene expression levels were normalized to hprt levels and are presented as a relative ratio compared to control levels. (A–K) represent: gene expression levels of (A) CD40; (B) CD80; (C) CD86; (D) CTLA-4; (E) MHC class I; (F) MHC class II; (G) IFNα; (H) IFNβ; (I) IFNγ; (J) IL-12p40; (K) IL-17A in porcine PBMCs were analyzed. The data represent the means ± SEM of triplicate measurements (n = 5/group). Statistical analyses were performed using one-way ANOVA with Tukey’s post-hoc test. *, # p < 0.05; **, ## p < 0.01; ***, ### p < 0.001.