Figure 4.

nAChR deficiency has no impact on the alveolar-capillary barrier or cytokine response in lung during S. pneumoniae infection after experimental stroke. MIP-1α, IL-10, KC and TNFα concentrations in BAL were measured one day after infection as described in Material and Methods. MIP-1α, IL-10, KC and TNFα level did not differ between groups (A–D). Positive correlation between MIP-1α, KC and TNFα concentrations and bacterial burden in BAL was found (Pearson Correlation, two tailed) (E). To investigate the effect of α2, α5, α7 and α9/10 nAChRs on the permeability of the alveolar-capillary barrier after MCAo, albumin concentrations in BAL and plasma were measured and the ratio of BAL albumin and plasma albumin was calculated. Albumin ratio is increased in several mice but does not differ between nAChR KO mice and WT mice (F). Data from 6 independent experiments are shown (n = 5–11 per group) as box plots compared to naïve WT mice as a reference group using the Kruskal–Wallis test followed by Dunn´s test for multiple comparison.