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. 2020 May 18;8(2):234. doi: 10.3390/vaccines8020234

Figure 7.

Figure 7

Clinical and virological parameters in experiment 2. Mean temperatures (A), clinical scores (B) up to six days and viremia up to five days post-challenge (C) of groups of six pigs primed and boosted with Antigen Pool A (blue), Antigen Pool C (red) or influenza NP (green). Temperatures (D,G,J) and clinical scores (E,H,K) for each individual pig immunized with influenza NP (D,E), Antigen Pool A (G,H) or Antigen Pool C (J,K) are shown. Blood was taken from animals immunized with Influenza NP (F), Antigen Pool A (I), Antigen Pool C (L) on the indicated days and DNA was extracted in duplicate and the viral titer determined by qPCR, with each extraction tested in duplicate. Data points are the mean of the duplicate extractions. Errors bars on panels A, B and C indicate standard deviation from the mean. Black asterisks on panels B and C show significant differences (repeated measures two-way ANOVA) of the indicated group of pigs compared to the control, * p ≤ 0.05 or ** p ≤ 0.01. Red asterisks on panels A and B show significant differences (repeated measures two-way ANOVA) between pigs immunized with Antigen Pools A and B six days post-infection, * p ≤ 0.05 or **** p ≤ 0.0001.