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. 2020 May 29;8(2):258. doi: 10.3390/vaccines8020258

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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UV-treatment of BinJ/WNV_KUN-prME does not affect authentic epitope presentation. (A) Infectious BinJ/WNV_KUN-prME titer in TCID₅₀/mL after 10, 20, 30, 40, 50 or 90 min of UV-C exposure. UV-treated vaccine was inoculated onto C6/36 and infectious virus titer was determined in fixed cell ELISA probed with 4G4. Dotted lines represent 50% and 99% reduction of infectious virus. Limits of detection are 2.3 log₁₀TCID₅₀/mL (lower limit) and 9.8 log₁₀TCID₅₀/mL (upper limit). (B) Epitope authenticity was determined by binding of monoclonal antibodies to prM and E domains and end-point dilutions were determined by ELISA on live versus UV-C inactivated chimeric virus antigen.