Figure 5.

Virus challenge with WNV_NY99 after vaccination with live of UV-C inactivated BinJ/WNV_KUN-prME vaccine. Male and female adult CD1 mice were challenged intraperitoneally with 10³ infectious units of WNV_NY99 at 21 days-post vaccination (n = 10; five males and five females). (A) Timeline of vaccination and bleeding. (B) Survival shown for all mice for 21 days-post virus challenge. (C) Mean viremias were determined by IPA on C6/36 cells, fixed at 2 dpi and probed with hE16 anti-WNV E [26]. Limit of detection was 1 log₁₀FFU/mL for individual serum samples. (D) Clinical signs of disease were collected for 21 days following infection by monitoring animals twice daily. Colors represent severity of clinical signs as a proportion of total animals in the group. Mouse weights were recorded daily and are represented as mean percentage of weight change from day of infection per group (G), or males (E) or females (F). Error bars represent SD of the mean.