Figure 2.

Intracellular localization of both Nef^mut/E7 and GC-AG Nef^mut/E7. Confocal microscope analysis of HEK-293T cells transfected with either Nef^mut, Nef^mut/E7, or GC-AG Nef^mut/E7 expressing vectors. Shown are representative fields from transfected cell cultures incubated first with an anti-Nef mAb, and then with Alexa 488-conjugated anti-mouse IgGs. DAPI (blue fluorescence) was used to highlight cell nuclei. Scale bars are reported. The results are representative of two independent experiments.