Figure 6.

HPV16-E7-specific CD8⁺ T-cell immunity induced in mice after i.m. DNA injection. CD8⁺ T-cell immune response in C57 Bl/6 mice inoculated i.m. with the DNA vectors expressing either Nef^mut/E7 or GC-AG Nef^mut/E7 DNA vectors. As controls, mice were inoculated with void vector. At the time of sacrifice, 2.5 × 10⁵ splenocytes were incubated o.n. with or without 5 μg/mL of either unrelated or E7-specific peptides in triplicate IFN-γ ELISpot microwells. Shown are the numbers of IFN-γ spot-forming units (SFU)/well as mean values of triplicates after subtraction of mean values measured in wells of splenocytes treated with the unspecific peptide. Reported are cumulate data from two independent experiments. Intragroup mean values + standard deviations are also reported.