Table 3.
Details of quantitative trait loci identified for Fusarium head blight response variables/traits disease incidence (DI), severity (DS), visual rating index (VRI = DI x DS), deoxynivalenol content (DON), and days to anthesis (DTA) and plant height (PHT) on different wheat chromosomes in AAC Innova x AAC Tenacious doubled haploid population using pooled data of different environments.
| Response Variable/ Trait |
Chr. (arm) | QTL Name a | QTL Position b | QTL Interval | LOD | Absolute Additive Effect | R 2 | Closest Marker | Individual Environment(s) | Favourable Donor Allele for Respective Trait | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Marker Name | Linkage Map Position | Physical Position (IWGSC RefSeq v2.0) c Start End |
|||||||||||
| DI | 2B | QFhi.lrdc-2B | 68.8 | 64.2–71.8 | 3.2 | 0.5 | 4.4 | BS00012078_51 | 66.73 | 265366661 | 265366634 | Mrd16, Mrd17 | T |
| 2DS | QFhi.lrdc-2D* | 40.9 | 40.2–63.7 | 15.5 | 1.0 | 25.0 | Ppd-D1 | 40.76 | 36205433 | 36204772 | Mrd15, Mrd16, Mrd17 | T | |
| 4A | QFhi.lrdc-4A | 118.0 | 114.8–121.6 | 8.0 | 0.7 | 11.9 | BobWhite_c10610_1096 | 117.82 | 688409042 | 688408942 | Mrd16, Mrd17 | I | |
| 4D | QFhi.lrdc-4D | 146.0 | 145.1–146.0 | 2.8 | 0.4 | 4.0 | BobWhite_c28101_376 | 145.98 | 515801764 | 515801671 | Mrd16, Mrd17 | I | |
| 5D | QFhi.lrdc-5D | 0.1 | 0.0–0.6 | 2.6 | 0.4 | 3.9 | RAC875_c92929_92 | 0.00 | 279491136 | 279491036 | Mrd17 | T | |
| 7A | QFhi.lrdc-7A | 206.4 | 205.9–206.8 | 2.6 | 0.4 | 2.1 | IACX2471 | 205.99 | 673715328 | 673715447 | -- | T | |
| DS | 2DS | QFhs.lrdc-2D.1* | 40.9 | 40.2–63.4 | 12.7 | 0.8 | 19.0 | Ppd-D1 | 40.76 | 36205433 | 36204772 | Mrd15, Mrd16, Mrd17 | T |
| 2DL | QFhs.lrdc-2D.2* | 115.6 | 105.2–126.4 | 5.4 | 0.5 | 15.2 | BobWhite_c17782_194 | 115.38 | 555098707 | 555098805 | Mrd15, Mrd16 | T | |
| 4A | QFhs.lrdc-4A | 118.0 | 114.8–121.3 | 5.4 | 0.5 | 10.1 | BobWhite_c10610_1096 | 117.82 | 688409042 | 688408942 | Mrd15, Mrd16, Mrd17 | I | |
| 7BS | QFhs.lrdc-7B | 32.3 | 24.3–35.5 | 3.6 | 0.4 | 6.3 | wsnp_CAP7_c44_26549 | 32.15 | 17091249 | 17091049 | Mrd16 | I | |
| VRI | 2DS | QFhb.lrdc-2D.1* | 40.9 | 40.2–63.4 | 12.4 | 9.4 | 18.0 | Ppd-D1 | 40.76 | 36205433 | 36204772 | Mrd15, Mrd16, Mrd17 | T |
| 2DL | QFhb.lrdc-2D.2* | 114.6 | 106.4–120.8 | 3.6 | 4.8 | 12.2 | BobWhite_c17782_194 | 115.38 | 555098707 | 555098805 | Mrd15 | T | |
| 4A | QFhb.lrdc-4A | 118.0 | 114.7–121.3 | 7.1 | 6.6 | 11.5 | BobWhite_c10610_1096 | 117.82 | 688409042 | 688408942 | Mrd15, Mrd16, Mrd17 | I | |
| 5D | QFhb.lrdc-5D | 0.2 | 0.0–0.4 | 2.5 | 3.4 | 3.0 | RAC875_c92929_92 | 0.00 | 279491136 | 279491036 | Mrd17 | T | |
| 7BS | QFhb.lrdc-7B | 31.6 | 24.1–35.2 | 3.9 | 4.9 | 7.3 | wsnp_CAP7_c44_26549 | 32.15 | 17091249 | 17091049 | Mrd16 | I | |
| DON | 2DS | QDon.lrdc-2D.1* | 40.6 | 37.9–41.9 | 3.9 | 3.0 | 12.3 | Ppd-D1 | 40.76 | 36205433 | 36204772 | Mrd16, Mrd17 | T |
| 2DL | QDon.lrdc-2D.2* | 112.6 | 105. 1–126.6 | 14.4 | 6.0 | 34.5 | BobWhite_c17782_194 | 115.38 | 555098707 | 555098805 | Mrd15, Mrd16, Mrd17 | T | |
| 3B | QDon.lrdc-3B.1* | 132.8 | 132.2–133.2 | 2.7 | 2.1 | 9.3 | Kukri_c13345_481 | 132.75 | 753508932 | 753508832 | Mrd15 | T | |
| 3B | QDon.lrdc-3B.2 | 147.3 | 144.5–150.3 | 2.7 | 2.3 | 4.0 | Excalibur_rep_c97324_623 | 151.49 | 771943609 | 771943709 | -- | T | |
| 4B | QDon.lrdc-4B* | 49.5 | 46.4–52.4 | 6.6 | 3.6 | 8.0 | Ex_c101685_705 | 49.48 | 31725418 | 31725518 | Mrd17 | T | |
| 5A | QDon.lrdc-5A.1 | 164.8 | 156.4–169.0 | 3.1 | 2.3 | 3.0 | IACX2540 | 164.62 | 621553818 | 621553699 | Mrd16, Mrd17 | T | |
| 7BS | QDon.lrdc-7B* | 52.9 | 52.2–57.7 | 3.4 | 2.7 | 5.0 | Kukri_c19823_491 | 52.69 | 60918162 | 60918262 | Mrd15 | I | |
| DTA | 2B | QDta.lrdc-2B | 65.9 | 64.9–66.7 | 2.6 | 0.6 | 3.0 | BS00065276_51 | 66.14 | 57526894 | 57526994 | Mrd17, Let18 | I |
| 2DS | QDta.lrdc-2D.1 | 41.9 | 40.2–63.5 | 22.9 | 2.1 | 30.0 | RAC875_c7319_195 | 41.83 | 36481727 | 36481627 | Mrd17, Let17, Let18, Let19 | I | |
| 4A | QDta.lrdc-4A.2 | 120.0 | 117.4–123.8 | 6.8 | 1.1 | 7.0 | Excalibur_c4325_1440 | 121.65 | 686650643 | 686650543 | Mrd17, Let18, Let19 | T | |
| 7BS | QDta.lrdc-7B | 27.3 | 18.1–31.9 | 6.6 | 1.1 | 8.0 | IACX198 | 24.26 | 10548520 | 10548633 | Mrd17, Let17, Let18, Let19 | T | |
| 7D | QDta.lrdc-7D.1 | 79.9 | 77.8–91.5 | 2.78 | 1.2 | 5.8 | D_GCE8AKX02ILA1U_88 | 79.8186 | 56638676 | 56638464 | Let17, Let18, Let19 | T | |
| 7D | QDta.lrdc-7D.2 | 117.7 | 113.0–118.0 | 3.4 | 0.8 | 3.5 | wsnp_Ex_c10430_17064001 | 118.58 | 114113970 | 114114170 | Mrd17 | T | |
| PHT | 1B | QPht.lrdc-1B.1 | 36.0 | 33.0–38.5 | 7.1 | 2.4 | 6.0 | IAAV4702 | 34.94 | 544295939 | 544296139 | Mrd17, LetPGH17 | I |
| 2DS | QPht.lrdc-2D.1 | 41.9 | 40.2–63.5 | 11.1 | 3.2 | 10.0 | RAC875_c7319_195 | 41.83 | 36481727 | 36481627 | Mrd17, Let17, Let18, Let19 | I | |
| 4A | QPht.lrdc-4A.1 | 22.6 | 17.8–26.8 | 2.6 | 1.5 | 2.0 | BS00092244_51 | 26.38 | 11756178 | 11756078 | Mrd17 | T | |
| 4B | QPht.lrdc-4B | 49.6 | 46.0–52.6 | 42.0 | 7.3 | 53.0 | Ex_c101685_705 | 49.48 | 31725418 | 31725518 | Mrd17, Let17, LetPGH17, Let18, Let19 | I | |
| 5A | QPht.lrdc-5A.2 | 97.8 | 91.9–103.0 | 3.7 | 1.7 | 3.0 | RAC875_c25072_389 | 97.75 | 524944276 | 524944376 | Let17, Let18 | T | |
| 5D | QPht.lrdc-5D | 26.5 | 24.7–42.5 | 2.9 | 1.7 | 3.0 | wsnp_JD_c3690_4731341 | 26.59 | 401527745 | 401527907 | -- | T | |
| 7D | QPht.lrdc-7D.1 | 79.9 | 67.9–95.2 | 5.0 | 2.0 | 4.0 | D_GCE8AKX02ILA1U_88 | 79.82 | 56638676 | 56638464 | Mrd17, Let17, Let18, Let19 | T | |
Note: Chr.: chromosome; QTL: quantitative trait loci; LOD: logarithm of the odds score; R2: percent of the phenotypic variance; Mrd: Morden; Let: Lethbridge; PGH: green house; 17: 2017; 18: 2018; 19: 2019; a QTL were named according to all loci identified using pooled (average of all environments) as well as individual environment phenotypic data; b QTL positions were determined by the highest LOD peak positions (cM) on linkage groups; c Physical positions were determined by SNP probe sequence mapped to reference genome; -- QTL detected using pooled data only; I = AAC Innova; T = AAC Tenacious; * loci also appeared during epistasis QTL analysis.