Skip to main content
. 2020 Jun 24;21(12):4482. doi: 10.3390/ijms21124482

Figure 4.

Figure 4

Reporter gene assay for the functional analysis of 3′-UTR regulated by miRNA. (A) The reporter construct contained the inserted DNA fragment of human SLC30A3 3′-UTR wild type (WT) or mutant (Mut) downstream of luc2 in the pmirGLO Dual-Luciferase miRNA Target Expression Vector. (B) Reporter gene assay was performed using CHO cells co-transfected with NC or miR-5572 mimic, and the reporter vector cloned with WT 3′-UTR of SLC30A3 or mutant 3′-UTR of SLC30A3 (n = 3 in each experimental group). All data are presented as box and scatter plot. Statistical significance was determined by two-way ANOVA followed by post hoc Bonferroni’s test (* p < 0.05).