Figure 3.

Immunogenicity of the Sf9-produced Nc-aD VLPs in BALB/c mice. ELISA was performed to determine the immunogenicity of the Sf9-produced Nc-aD VLPs and the control groups. The ELISA plate was coated with a synthetic aD peptide, and reacted with serum samples from the immunised mice. Mice were acclimatised from day 0 to 14 and immunised with the primary injection on day 14. The first and second booster doses were administered on days 35 and 56, respectively. The letters (a, b, c, d, A, B, C, i, J, k, o, x, y, z) and symbols (I, II, III, ‡, ** ) represent the statistical significance of the measurements at each time point. Measurements marked by different letters/symbols are significantly different (p < 0.001), and those marked by the same letters/symbols are not significantly different. The error bars indicate the standard deviation (SD) of triplicate measurements.